Pulmonary Toxicity of Inhaled Diesel Exhaust and Carbon Black in Chronically Exposed Rats. Part III: Examination of Possible Target Genes

Research Report 68-III,

In Part III of this study, Dr. Belinsky and his associates at the Lovelace Biomedical and Environmental Research Institute examined lung tumors from rats that had inhaled high concentrations of diesel engine exhaust or carbon black particles (see Part I by Dr. Joe Mauderly). The investigators applied molecular biology techniques to measure mutations in selected genes in the DNA from the tumors. Mutations in portions of the K-ras protooncogene and the p53 tumor suppressor gene were targeted for analysis because patterns of mutations in these genes previously have been associated with exposure to carcinogens in laboratory animals and humans.