Pulmonary Toxicity of Inhaled Diesel Exhaust and Carbon Black in Chronically Exposed Rats. Part II: DNA Damage

Dr. Randerath's study was part of a large cancer bioassay conducted by Dr. Joe Mauderly and colleagues of the Inhalation Toxicology Research Institute (ITRI). The investigators exposed F344/N rats by inhalation to clean (filtered) air or to one of two concentrations of either diesel exhaust or carbon (2.5 or 6.5 mg of particles/m3 of test atmosphere). Both Dr. Randerath and Dr. Mauderly measured DNA adducts in lung tissue samples from rats exposed at ITRI for different periods of time to the test atmospheres. Dr. Randerath and colleagues also extracted the organic compounds from the diesel exhaust particles, applied the diesel exhaust extract to the skin of CD-1 mice, and looked for DNA adducts in the skin, lung, and heart tissues of these mice.