Oxidant Effects on Rat and Human Lung Proteinase Inhibitors

Research Report 37,

This report describes a study by Dr. Johnson and colleagues to test the hypothesis that inhaled oxidants can cause lung damage by inactivating the proteinase inhibitors that normally protect the lung from proteolysis. In the first set of experiments, the functional activity of rat alpha-1-proteinase inhibitor (á1-PI) was measured in rat lung lavage fluid from rats exposed acutely or chronically to varying concentrations of NO2, diesel exhaust, O3, and O3 in conjunction with CO2. In the final set of experiments, functional activity of á1-PI was measured in the bronchoalveolar lavage fluid of human volunteers exposed acutely to NO2 or O3.