Effects of Ozone and Nitrogen Dioxide on Human Lung Proteinase Inhibitors

Research Report 11, August 1987

Addressing the need for better assessment of human exposure to mobile source emissions, this report investigates proteinase inhibitor activity as a potential biomarker of oxidant exposure. In this study by Dr. Johnson, human participants were exposed to 0.5 ppm ozone for four hours on consecutive days and to concentrations ranging from 0.6-2 ppm nitrogen dioxide for three hours. Blood samples were obtained and the functional activity of the proteinase inhibitors, alpha-1-proteinase, and bronchial leukocyte proteinase was assessed. Subsequently, in vitro nitrogen dioxide exposure studies were conducted to compare differences in substrate inhibition by oxidized inhibitors.