DNA Adduct Formation and T-Lymphocyte Mutation Induction in F344 Rats Implanted with Tumorigenic Doses of 1,6-Dinitropyrene

Dr. Beland and his associates at the University of Arkansas School of Medical Sciences developed an assay to measure mutations induced by dinitropyrenes, a class of diesel engine exhaust, in rats. The investigators analyzed the mutations in a selected gene in spleen T lymphocytes from rats treated with 1,6-dinitropyrene under conditions that induced lung tumors at the highest dose tested. They also examined DNA adduct levels in lung and liver tissues and in spleen lymphocytes and white blood cells.